Why are variants identified in a single strand considered as artifacts, in particular when using amplicon kits?
Please see the following Technical Note from Illumina:
![illumina](https://docs.varsome.com/hubfs/illumina.png)
Each amplicon is the PCR product from one DNA strand, but since DNA is double stranded, a real variant is supposed to be found in both stands:
![illumina2](https://docs.varsome.com/hubfs/illumina2.png)